AtpH anticorps

N° du produit ABIN249344

L’anticorps Lapin Polyclonal anti- a été validé pour WB. Il convient pour détecter dans des échantillons de Arabidopsis thaliana, Nicotiana tabacum, Spinach et Thermosynechococcus elongatus.

Aperçu rapide pour AtpH anticorps (ABIN249344)

Antigène: AtpH

Reactivité: Arabidopsis thaliana, Nicotiana tabacum, Spinach, Thermosynechococcus elongatus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Specificité: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 8 kDa (for Arabidopsis thaliana)

Purification: serum

Immunogène: purified c subunit from Spinacia oleracea P69447

Information d'application

Indications d'application: 1: 1000 - 1: 10 000 with standard ECL (WB)

Commentaires:

please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détail du antigène

Antigène: AtpH

Sujet: AGI Code: ATCG00140
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.

Poids moléculaire: 8 kDa (for Arabidopsis thaliana)

UniProt: P69447